Long-term and short-term effects of abscisic acid on plantlet acclimatization to ex vitro conditions

0339833 - ÚEB 2010 RIV CU eng C - Conference Paper (international conference)
Pospíšilová, Jana - Haisel, Daniel - Synková, Helena - Baťková, Petra
Long-term and short-term effects of abscisic acid on plantlet acclimatization to ex vitro conditions.
Proceedings of "Congreso International de Biotecnología Vegetal". Ciego de Avila: University of Ciego, 2009, s. 1-6.
[Congreso International de Biotecnología Vegetal. Congreso International de Biotecnología Vegetal (ES), 05.10.2009-07.10.2009]
R&D Projects: GA ČR GA522/07/0227
Institutional research plan: CEZ:AV0Z50380511
Keywords : carotenoids * chlorophyll contents * chlorophyll fluorescence
Subject RIV: ED - Physiology

We applied ABA either to the last subculture (3 weeks; 10 mM ABA) or as single application (50 mM ABA) immediately after ex vitro transfer of tobacco plantlets. Transplanted plants were grown either under low (LI) or high (HI) irradiance of 150 and 700 mmol m-2 s-1, respectively. Endogenous content of ABA increased considerably after ABA application. Stomatal conductance, transpiration rate and net photosynthetic rate decreased considerably by ABA application and wilting of these plants was limited. Water use efficiency markedly increased due to ABA application. Chlorophyll a+b content was higher in ABA-treated plants. While long-term ABA application mainly decreased content of xanthophylls, short-term application decreased particularly degree of their deepoxidation. Both long-term and short-term applications of ABA improved acclimatization of in vitro grown plants to ex vitro conditions.
Permanent Link: http://hdl.handle.net/11104/0183241