Comparison of in vitro tests for measurement of estrogenity of chlorinated compounds and their use in the evaluation of biodegradation of chlorbenzoates by the fungus Panus tigrinus

0339618 - MBÚ 2010 RIV CZ eng C - Conference Paper (international conference)
Svobodová, Kateřina - Covino, S. - Křesinová, Zdena - Cajthaml, Tomáš
Comparison of in vitro tests for measurement of estrogenity of chlorinated compounds and their use in the evaluation of biodegradation of chlorbenzoates by the fungus Panus tigrinus.
Odpadové fórum 2009 - sborník přednášek. Praha: PCHE-PetroChemEng, 2009, s. 3619-3623. ISBN 978-80-02-02108-7.
[Odpadové fórum. Milovy (CZ), 22.04.2009-24.04.2009]
R&D Projects: GA MŠMT 2B06156
Institutional research plan: CEZ:AV0Z50200510
Keywords : yeast assay * biodegradation * chlorbenzoates
Subject RIV: EE - Microbiology, Virology

Recently, contamination of the environment by endocrine disruptive compounds, toxicity of these chemicals and their biodegradability are widely studied. This work is focused on estrogenity of chlorinated phenols and chlorbenzoic acids that represent potential degradation products of PCB compounds. Two different yeast bioassays for evaluation of estrogenity based on recombinated strains of Saccharomyces cerevisie were compared in this work. Estrogenic activity of chlorbenzoic acids varied with the chemical structure of chemicals. A mixture of chlorbenzoates (initial concentration 10 ppm) was degraded by a ligninolytic fungus Panus tigrinus in liquid cultures. Chemicals resulting from degradation were tested for their estrogenity, however, results indicated that the test responses were inhibited probably by some compounds present in the fungal culture liquid
Permanent Link: http://hdl.handle.net/11104/0005744