Sperm antibodies, intra-acrosomal sperm proteins, and cytokines in semen in men from infertile couples

0339340 - BTÚ 2010 RIV US eng J - Journal Article
Ulčová-Gallová, Z. - Gruberová, J. - Vrzalová, J. - Bibková, K. - Pěknicová, Jana - Mičanová, Z. - Topolčan, O.
Sperm antibodies, intra-acrosomal sperm proteins, and cytokines in semen in men from infertile couples.
American Journal of Reproductive Immunology. Roč. 61, č. 3 (2009), s. 236-245. ISSN 1046-7408. E-ISSN 1600-0897
Institutional research plan: CEZ:AV0Z50520701
Keywords : intra-acrosomal protein * cytokines * infertility
Subject RIV: EC - Immunology
Impact factor: 2.172, year: 2009

The aim of this study was to investigate seminal sperm-agglutinating antibodies, intra-acrosomal proteins, sperm head abnormalities, and cytokines in men from infertile couples.The direct mixed anti-immunoglobulin reaction test in semen, and immunocytochemical method using monoclonal antibodies and immunofluorescence for the examination of intra-acrosomal proteins in the spermatozoa were used. Cytokines in seminal plasma were determined by multiplex immunoanalytic xMAP. Sperm-agglutinating antibodiesin seminal plasma were found to be more in astheno- and oligoasthenospermatic men than in normospermatic men. Sperm head pathology and very low amounts of acrosomal proteins were frequently detected in pathologic semen samples. Cytokine levels defined as 'high' were obtained especially for IL-8, IL-5, IL-6,IL-10. The high cellularity in semen was correlated with higher IL-5. Immunologic cause of male infertility is a very important risk factor in the pathogenesis of sperm cells.
Permanent Link: http://hdl.handle.net/11104/0182895