Differentiation between activity of digestive enzymes of .i.Brachionus calyciflorus./i. and extracellular enzymes of its epizooic bacteria

0338890 - BC 2010 RIV IT eng J - Journal Article
Štrojsová, Martina - Ahlrichs, W.H.
Differentiation between activity of digestive enzymes of Brachionus calyciflorus and extracellular enzymes of its epizooic bacteria.
Journal of Limnology. Roč. 68, č. 2 (2009), s. 409-412. ISSN 1129-5767. E-ISSN 1723-8633
Institutional research plan: CEZ:AV0Z60170517
Keywords : rotifers * phosphatase * beta-N-acetylhexosaminidase * enzyme localization
Subject RIV: EG - Zoology
Impact factor: 0.932, year: 2009

The rotifer Brachionus calyciflorus was examined by scanning electron microscopy (SEM) for surface-attached, i.e. epizootic, bacteria to ascertain their specific localization and thus find out if we could discern between rotifer and bacterial enzyme activity. The lorica of B. calyciflorus was colonized by one distinct type of bacteria, which originated from the algal culture used for rotifer feeding. The corona, posterior epidermis and foot of all inspected individuals were always without attached bacteria. The density of the attached bacteria was higher with the increasing age of B. calyciflorus. We hypothesize that epizooic bacteria may produce the ectoenzymes phosphatases and beta-N-acetylhexosaminidases on the lorica, but not on the corona of B. calyciflorus. Since enzyme activities of epizooic bacteria may influence the values and interpretation of bulk rotifer enzyme activities, we should take the bacterial contribution into account.
Permanent Link: http://hdl.handle.net/11104/0182547