Identification of molecular targets for selective elimination of TRAIL-resistant leukemia cells. From spots to in vitro assays using TOP15 charts

0338760 - MBÚ 2010 RIV DE eng J - Journal Article
Petrák, J. - Toman, O. - Šimonová, T. - Halada, Petr - Čmejla, R. - Klener, P. - Živný, J.
Identification of molecular targets for selective elimination of TRAIL-resistant leukemia cells. From spots to in vitro assays using TOP15 charts.
Proteomics. Roč. 9, č. 22 (2009), s. 5006-5015. ISSN 1615-9853. E-ISSN 1615-9861
Institutional research plan: CEZ:AV0Z50200510
Keywords : Biomedicine * Drug resistance * HL-60
Subject RIV: CE - Biochemistry
Impact factor: 4.426, year: 2009

The resistance of malignant cells to chemotherapy calls for the development of novel anti-cancer drugs. TNF-related apoptosis-inducing ligand (TRAIL) is a pro-apoptotic cytokine, which selectively induces apoptosis in malignant cells. We derived two TRAIL-resistant HL-60 subclones, HL-60/P1 and HL-60/P2, from a TRAIL-sensitive HL-60 acute promyelocytic leukemia cell line. To identify therapeutically exploitable weaknesses of the TRAIL-resistant leukemia cells that could be used as molecular targets for their elimination, we performed proteomic (2-DE) analysis and compared both TRAIL-resistant subclones with the original TRAIL-sensitive HL-60 cells. We identified over 40 differentially expressed proteins. To significantly narrow the lists of candidate proteins, we excluded proteins that are known to be often differentially expressed, regardless of experiment type and tissue (the so-called TOP15 proteins)
Permanent Link: http://hdl.handle.net/11104/0182449