Hepcidin, the Hormone of Iron Metabolism, is Bound Specifically to a-2-Macroglobulin

0338673 - MBÚ 2010 RIV US eng J - Journal Article
Pešlová, G. - Petrák, J. - Kuželová, K. - Hrdý, I. - Halada, Petr - Kuchel, P. W. - Soe-Lin, S. - Ponka, P. - Šuták, R. - Becker, E. - Huang, M. L.-H. - Rahmanto, Y. S. - Richardson, D. R. - Vyoral, D.
Hepcidin, the Hormone of Iron Metabolism, is Bound Specifically to a-2-Macroglobulin.
Blood. Roč. 113, č. 24 (2009), s. 6225-6236. ISSN 0006-4971. E-ISSN 1528-0020
R&D Projects: GA ČR(CZ) GD204/03/H066; GA MŠMT LC07017
Institutional research plan: CEZ:AV0Z50200510
Keywords : RECEPTOR-RELATED PROTEIN * GROWTH-FACTOR-BETA * BINDING-PROTEIN
Subject RIV: CE - Biochemistry
Impact factor: 10.555, year: 2009

Hepcidin is a major regulator of iron metabolism. Hepcidin-based therapeutics/diagnostics could play roles in hematology in the future, and thus, hepcidin transport is crucial to understand. In this study, we identify alpha2-macroglobulin (alpha2-M) as the specific hepcidin-binding molecule in blood. Interaction of 125I-hepcidin with alpha2-M was identified using fractionation of plasma proteins followed by native gradient polyacrylamide gel electrophoresis and mass spectrometry. Hepcidin binding to nonactivated alpha2-M displays high affinity , whereas hepcidin binding to albumin was nonspecific and displayed nonsaturable kinetics
Permanent Link: http://hdl.handle.net/11104/0182392