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Denaturing gradient gel electrophoresis as a fingerprinting method for the analysis of soil microbial communities

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    0338647 - MBÚ 2010 RIV CZ eng J - Journal Article
    Valášková, Vendula - Baldrian, Petr
    Denaturing gradient gel electrophoresis as a fingerprinting method for the analysis of soil microbial communities.
    Plant, Soil and Environment. Roč. 55, č. 10 (2009), s. 413-423. ISSN 1214-1178. E-ISSN 1805-9368
    R&D Projects: GA ČR GA526/08/0751; GA MŠMT LC06066
    Institutional research plan: CEZ:AV0Z50200510
    Keywords : DGGE * bacteria * fungi
    Subject RIV: EE - Microbiology, Virology
    Impact factor: 0.697, year: 2009

    Among the methods currently used to compare microbial communities based on nucleic acid sequences, the techniques based on differences in the melting properties of double-stranded molecules, denaturing gradient gel electrophoresis (DGGE) or temperature gradient gel electrophoresis (TGGE), are the most widely used. Their main advantage is that they provide the possibility to further analyze whole sequences contained in fingerprints using molecular methods
    Permanent Link: http://hdl.handle.net/11104/0182371

     
     
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