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Preparative divergent flow IEF without carrier ampholytes for separation of complex biological samples
- 1.0338379 - ÚIACH 2010 RIV DE eng J - Journal Article
Šťastná, Miroslava - Šlais, Karel
Preparative divergent flow IEF without carrier ampholytes for separation of complex biological samples.
Electrophoresis. Roč. 31, č. 3 (2010), s. 433-439. ISSN 0173-0835. E-ISSN 1522-2683
R&D Projects: GA AV ČR IAAX00310701
Institutional research plan: CEZ:AV0Z40310501
Keywords : carrier-free divergent flow IEF * proteins * yeast lysate
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 3.569, year: 2010
Based on the principles of recently newly established electrophoretic method called divergent flow IEF (DF IEF), we have tested the DF IEF instrument operating without the use of background carrier ampholytes. In DF IEF, the proteins are separated, desalted and concentrated in one step. We have shown that during separation and focusing of sample consisting of high numbers of proteins (yeast lysate and wheat flour extract), the pH gradient of preparative divergent flow IEF (DF IEF) can be created by autofocusing of the sample components themselves.
Permanent Link: http://hdl.handle.net/11104/0182167
Number of the records: 1