The content of beta-sitosterol in maize plants under different nitrogen nutrition

0338340 - ÚEB 2010 RIV US eng C - Conference Paper (international conference)
Pavlíková, D. - Pavlík, Milan - Balík, J.
The content of beta-sitosterol in maize plants under different nitrogen nutrition.
Proceedings of the International Plant Nutrition Colloquium XVI. California: University of California, 2009 - (Abadia, J.; Schjoerring, J.), s. 1-5
[International Plant Nutrition Colloquium /16./. Sacramento (US), 26.08.2009-30.08.2009]
R&D Projects: GA MZe 1G58027; GA MŠMT 2B06024; GA ČR GA521/09/1150
Institutional research plan: CEZ:AV0Z50380511
Keywords : sterols * phytosterols * HPLC
Subject RIV: CB - Analytical Chemistry, Separation
http://escholarship.org/uc/item/72j7v14x

The effect of nitrogen nutrition on phytosterol content in aboveground biomass of maize (Zea mays L.) was investigated in a pot experiment. For cultivation of maize plants nitrogen dose (2 or 4 g N per pot) was applied in the form of ammonium nitrate (AN) for control treatments or urea ammonium nitrate solution (UAN). UAN solution was applied according to the CULTAN method (Controlled Uptake Long Term Ammonium Nutrition). The lower nitrogen content in soil of UAN treatments in the first period of experiment and in following nitrogen application (30 days after maize sowing) affected yield and also total nitrogen content of aboveground biomass. The results of free ß-sitosterol analyses by HPLC showed the changes of its concentration after UAN application in contrast to AN treatments. The significant decrease of ß-sitosterol concentration (by 48 % in contrast to 1st period) was determined on UAN2 treatment in the 2nd period (8 days after nitrogen application).
Permanent Link: http://hdl.handle.net/11104/0182139