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Testing of the influenza virus purification by CIEF
- 1.0337690 - ÚIACH 2010 RIV DE eng J - Journal Article
Horká, Marie - Kubíček, O. - Kubesová, Anna - Kubíčková, Z. - Rosenbergová, K. - Šlais, Karel
Testing of the influenza virus purification by CIEF.
Electrophoresis. Roč. 31, č. 2 (2010), s. 331-338. ISSN 0173-0835. E-ISSN 1522-2683
R&D Projects: GA AV ČR IAAX00310701
Institutional research plan: CEZ:AV0Z40310501
Keywords : capillary isoelectric focusing * PCR * purification efficiency
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 3.569, year: 2010
In this contribution Equine and Swine influenza viruses from the infected allantoic fluid from specific pathogen free embryonated chicken eggs were precipitaded by PEG 6000, sodium chloride and the impurities with different densities were removed in the sucrose gradients. The purification efficiency of this technique and the isoelectric points of tested viruses were determined by capillary isoelectric focusing, the purification efficiencies were compared with the results from PCR.
Permanent Link: http://hdl.handle.net/11104/0181632
Number of the records: 1