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Determination and Quantification of Collagen Types in Tissues Using HPLC-MS/MS

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    0337646 - FGÚ 2010 RIV US eng J - Journal Article
    Pataridis, Statis - Eckhardt, Adam - Mikulíková, Kateřina - Sedláková, Pavla - Mikšík, Ivan
    Determination and Quantification of Collagen Types in Tissues Using HPLC-MS/MS.
    [Určení a kvantifikace kolagenních typů v tkáních pomocí HPLC-MS/MS.]
    Current Analytical Chemistry. Roč. 5, č. 4 (2009), s. 316-323. ISSN 1573-4110. E-ISSN 1875-6727
    R&D Projects: GA ČR GA203/09/0675; GA ČR(CZ) GA203/08/1428; GA MŠMT(CZ) 1M0510
    Institutional research plan: CEZ:AV0Z50110509
    Keywords : collagen * proteomics
    Subject RIV: CB - Analytical Chemistry, Separation
    Impact factor: 2.143, year: 2009

    There was developed method suitable for the determination of five collagen types in biological tissues (placenta, cartilage). This method is based on the salt precipitation of collagens, their cleavage by CNBr and trypsin, and analysis of specific peptides by HPLC-MS/MS

    Byla vyvinuta metoda umožňující stanovit pět kolagenních typů v biologických tkáních (placenta, chrupavka). Metoda je založena na precipitaci kolagenu solí, štěpení pomocí CNBr a trypsinu a následné analýze specifických peptidů pomocí HPLC-MS/MS
    Permanent Link: http://hdl.handle.net/11104/0181595

     
     
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