Nebularine Affects Plant Growth and Development but does not Interfere with Cytokinin Signaling

0337568 - ÚEB 2010 RIV US eng J - Journal Article
Pospíšilová, H. - Nisler, Jaroslav - Spíchal, Lukáš - Frébort, Ivo
Nebularine Affects Plant Growth and Development but does not Interfere with Cytokinin Signaling.
Journal of Plant Growth Regulation. Roč. 28, č. 4 (2009), s. 321-330. ISSN 0721-7595. E-ISSN 1435-8107
R&D Projects: GA ČR(CZ) GA522/06/0022; GA ČR GD522/08/H003; GA ČR(CZ) GP522/07/P197
Institutional research plan: CEZ:AV0Z50380511
Keywords : Anticytokinin * Arabidopsis thaliana * Cytokinin receptor
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.438, year: 2009

Nebularine is known for its high cytotoxicity in animals, whereas in plants it was originally believed to be an anticytokinin. In this study we show that in classical cytokinin bioassays, nebularine antagonized cytokinin function in senescence and callus biotests but not in the Amaranthus bioassay. Nebularine reversed the inhibitory effect of cytokinin on lateral root formation in Arabidopsis seedlings, and when applied alone caused increased lateral root formation and shortening of the main root. Systematic spraying of Arabidopsis plants with nebularine led to yellowing and formation of purple pigments, local drying, and withering, although younger plants showed a greater resilience. Comparison of nebularine cytotoxicity in plant and animal cells showed that the growth of tobacco BY-2 cells was inhibited with only about tenfold lower efficacy than mammalian cell lines.
Permanent Link: http://hdl.handle.net/11104/0181531