CHARACTERIZATION OF LOW-MOLECULAR WEIGHT COLLAGEN HYDROLYSATES PREPARED BY COMBINATION OF ENZYMATIC AND ACID HYDROLYSIS

0337491 - ÚEB 2010 RIV US eng J - Journal Article
Kašpárková, V. - Kolomazník, K. - Burketová, Lenka - Šašek, Vladimír - Šimek, L.
CHARACTERIZATION OF LOW-MOLECULAR WEIGHT COLLAGEN HYDROLYSATES PREPARED BY COMBINATION OF ENZYMATIC AND ACID HYDROLYSIS.
Journal of the American Leather Chemists Association. Roč. 104, č. 2 (2009), s. 46-51. ISSN 0002-9726
R&D Projects: GA MZe QH72117
Institutional research plan: CEZ:AV0Z50380511
Keywords : MODIFIED GELATIN * CHROME SHAVINGS * WASTE
Subject RIV: GF - Plant Pathology, Vermin, Weed, Plant Protection
Impact factor: 0.564, year: 2009

Formic, phosphoric and nitric acids were used for hydrolysis of the commercially available hydrolysis product of chrome shavings Hykol-E. The aim of the work was to prepare low-molecular weight product (M-w < 5000 g.mol(-1)) to be used as the plant bio-stimulator. Course of hydrolysis was monitored by following the changes of molecular weight (MW) and molecular weight distribution (MWD) determined by gel permeation chromatography (GPC). The study demonstrated that MW and MWD of hydrolysates are influenced by the choice of hydrolysing acid, its concentration as well as by the time of acid treatment. Using the combination of enzymatic and acid hydrolysis, it is possible to prepare products with the tailor-made, pre-defined molecular weight fitting the intended use.
Permanent Link: http://hdl.handle.net/11104/0181471