Effect of Potato Virus Y on the NADP-Malic Enzyme from Nicotiana tabacum L.: mRNA, Expressed Protein and Activity

0337489 - ÚEB 2010 RIV US eng J - Journal Article
Doubnerová, V. - Müller, Karel - Čeřovská, Noemi - Synková, Helena - Spoustová, Petra - Ryšlavá, H.
Effect of Potato Virus Y on the NADP-Malic Enzyme from Nicotiana tabacum L.: mRNA, Expressed Protein and Activity.
International Journal of Molecular Sciences. Roč. 10, č. 8 (2009), s. 3583-3598. E-ISSN 1422-0067
R&D Projects: GA MŠMT 1M0505
Institutional research plan: CEZ:AV0Z50380511
Keywords : NADP-malic enzyme * Nicotiana tabacum L. * Potato virus Y strain NTN (PVYNTN)
Subject RIV: EI - Biotechnology ; Bionics
Impact factor: 1.387, year: 2009

The effect of biotic stress induced by viral infection ( Potato virus Y, strain NTN and O) on NADP-malic enzyme (EC 1.1.1.40) in tobacco plants ( Nicotiana tabacum L., cv. Petit Havana, SR1) was tested at the transcriptional, translational and activity level. The increase of enzyme activity in infected leaves was correlated with the increased amount of expressed protein and with mRNA of cytosolic NADP-ME isoform. Transcription of the chloroplastic enzyme was not influenced by viral infection. The increase of the enzyme activity was also detected in stems and roots of infected plants. The effect of viral infection induced by Potato virus Y, NTN strain, causing more severe symptoms, was compared with the effect induced by milder strain PVYO. The observed increase in NADP-malic enzyme activity in all parts of the studied plants was higher in the case of PVYNTN strain than in the case of strain PVYO. The relevance of NADP-malic enzyme in plants under stress conditions was discussed.
Permanent Link: http://hdl.handle.net/11104/0181469