Analysis of mRNA Associated factors During Bovine Oocyte Maturation and Early Embryonic Development

0337303 - ÚŽFG 2010 RIV US eng J - Journal Article
Siemer, C. - Smiljakovic, T. - Bhojawni, M. - Leiding, C. - Kanitz, W. - Kubelka, Michal - Tomek, W.
Analysis of mRNA Associated factors During Bovine Oocyte Maturation and Early Embryonic Development.
Molecular Reproduction and Development. Roč. 76, č. 12 (2009), s. 1208-1219. ISSN 1040-452X. E-ISSN 1098-2795
R&D Projects: GA ČR GA524/07/1087
Institutional research plan: CEZ:AV0Z50450515
Keywords : In Vitro maturation * Poly(A) binding protein * Initiation-factor 4E
Subject RIV: ED - Physiology
Impact factor: 2.041, year: 2009

Regulation of gene expression at the translational level is particularly essential during developmental periods, when transcription is impaired. According to the closed-loop model of translational initiation, we have analyzed components of the 5'-mRNA cap-binding complex eIF4F (eIF4E, eIF4G, eIF4A), the eIF4E repressor 4E-BP1, and 3'-mRNA poly-(A) tail-associated proteins (PABP1 and 3, PAIP1 and 2, CPEB1, Maskin) during in vitro maturation of bovine oocytes and early embryonic development up to the 16-cell stage. Furthermore, we have elucidated the activity of distinct kinases which are potentially involved in their phosphorylation. Major phosphorylation of specific target sequences of PKA, PKB, PKC, CDKs, ATM/ATR, and MAPK were observed in M II stage oocytes. Furthermore, main changes in the abundance and/or phosphorylation of distinct mRNA-binding factors occur at the transition from M II stage oocytes to 2-cell embryos.
Permanent Link: http://hdl.handle.net/11104/0181329