Phospholipase D family interactions with the cytoskeleton: isoform delta promotes plasma membrane anchoring of cortical microtubules

0337286 - ÚEB 2010 RIV US eng J - Journal Article
Andreeva, Z. - Ho, A. Y. Y. - Barthet, M. M. - Potocký, Martin - Bezvoda, R. - Žárský, Viktor - Marc, J.
Phospholipase D family interactions with the cytoskeleton: isoform delta promotes plasma membrane anchoring of cortical microtubules.
Functional Plant Biology. Roč. 36, č. 7 (2009), s. 600-612. ISSN 1445-4408. E-ISSN 1445-4416
R&D Projects: GA AV ČR IAA601110916
Institutional research plan: CEZ:AV0Z50380511
Keywords : Allium * Arabidopsis * F-actin-microtubule interactions
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.678, year: 2009

Phospholipase D (PLD) is a key enzyme in signal transduction-mediating plant responses to various environmental stresses including drought and salinity. Isotype PLD delta interacts with the microtubule cytoskeleton, although it is unclear if, or how, each of the 12 PLD isotypes in Arabidopsis may be involved mechanistically. We employed RNA interference in epidermal cells of Allium porrum L. (leek) leaves, in which the developmental reorientation of cortical microtubule arrays to a longitudinal direction is highly sensitive to experimental manipulation. Using particle bombardment and transient transformation with synthetic siRNAs targeting AtPLD alpha, beta, gamma, delta, epsilon and zeta, we examined the effect of 'cross-target' silencing orthologous A. porrum genes on microtubule reorientation dynamics during cell elongation. Co-transformation of individual siRNAs together with a GFP-MBD microtubule-reporter gene revealed that siRNAs targeting AtPLD delta promoted, whereas siRNAs targeting AtPLD.
Permanent Link: http://hdl.handle.net/11104/0181314