Phospholipase D Activation Is an Early Component of the Salicylic Acid Signaling Pathway in Arabidopsis Cell Suspensions

0337283 - ÚEB 2010 RIV US eng J - Journal Article
Krinke, Ondřej - Flemr, M. - Vergnolle, Ch. - Collin, S. - Renou, J. P. - Taconnat, L. - Yu, A. - Burketová, Lenka - Valentová, O. - Zachowski, A. - Ruelland, E.
Phospholipase D Activation Is an Early Component of the Salicylic Acid Signaling Pathway in Arabidopsis Cell Suspensions.
Plant Physiology. Roč. 150, č. 1 (2009), s. 424-436. ISSN 0032-0889. E-ISSN 1532-2548
R&D Projects: GA ČR GA203/05/0559; GA MŠMT(CZ) LC06034
Institutional research plan: CEZ:AV0Z50380511
Keywords : SYSTEMIC ACQUIRED-RESISTANCE * TRANSCRIPTION FACTOR * INNATE IMMUNITY
Subject RIV: CE - Biochemistry
Impact factor: 6.235, year: 2009

Salicylic acid (SA) plays a central role in defense against pathogen attack, as well as in germination, flowering, senescence, and the acquisition of thermotolerance. In this report we investigate the involvement of phospholipase D (PLD) in the SA signaling pathway. In presence of exogenous primary alcohols, the production of phosphatidic acid by PLD is diverted toward the formation of phosphatidylalcohols through a reaction called transphosphatidylation. By in vivo metabolic phospholipid labeling with P-33(i), PLD activity was found to be induced 45 min after addition of SA. We show that incubation of Arabidopsis (Arabidopsis thaliana) cell suspensions with primary alcohols inhibited the induction of two SA-responsive genes, PATHOGENESIS-RELATED1 and WRKY38, in a dose-dependent manner. This inhibitory effect was more pronounced when the primary alcohols were more hydrophobic. Secondary or tertiary alcohols had no inhibitory effect.
Permanent Link: http://hdl.handle.net/11104/0181312