Cloning and Molecular Analyses of Hop Transcription Factors

0336689 - BC 2010 RIV BE eng C - Conference Paper (international conference)
Matoušek, Jaroslav - Kocábek, Tomáš - Škopek, Josef - Orctová, Lidmila - Stehlík, Jan - Füssy, Zoltán - Patzak, J. - Krofta, K. - Maloukh, L. - Roldán-Ruiz, I. - Heyerick, A. - De Keukeleire, D.
Cloning and Molecular Analyses of Hop Transcription Factors.
Proceedings of the Second International Humulus Symposium. Ghent: ISHA Acta Horticulturae, 2009 - (DeKeukeleire, D.; Hummer, K.; Heyerick, A.), s. 41-48. ISBN 978-90-6605-722-7. ISSN 0567-7572.
[ISHS International Humulus Symposium /2./. Ghent (BE), 01.09.2009-05.09.2009]
R&D Projects: GA ČR GA521/08/0740; GA MZe QH81052; GA ČR(CZ) GP521/06/P323
Institutional research plan: CEZ:AV0Z50510513
Keywords : transcription regulators * cis-elements * hop metabolome
Subject RIV: EB - Genetics ; Molecular Biology

Expression of hop chs_H1 and other hop chalcon synthase (CHS)-like enzymes was assayed in developing pollen tissue. Both chs_H1 and valerophenone synthase (vps) are highly expressed in pollen suggesting the existence of common regulatory element(s). While vps is specific for female and male floral tissues, the oligofamily of chs_H1 is expressed in both generative and somatic tissues, suggesting a more universal promoter. Novel transcription factors (TFs) from bZIP and bHLH families involved in chs_H1 regulation have been found by means of genomic and cDNA libraries screening. These include HlbZIP1A and its truncated variant HlbZIP1B, HlbZIP2 and HlbHLH. The activation potential of HlbZIP2 and previously cloned hop TFs were analysed in P.hybrida and N.benthamiana transient expression systems. HlbZIP2, as well as both HlMyb1 and HlMyb3 were found as activators of chs_H1 promoter. In combinatorial system, HlbZIP2 was found to modulate the expression of both Myb TFs from hop.
Permanent Link: http://hdl.handle.net/11104/0180873