Gene Specific Molecular Markers for Hop (Humulus lupulus L.)

0336095 - BC 2010 RIV BE eng C - Conference Paper (international conference)
Patzak, J. - Matoušek, Jaroslav
Gene Specific Molecular Markers for Hop (Humulus lupulus L.).
Proceedings of the Second International Humulus Symposium. Ghent: ISHA Acta Horticulturae, 2009 - (DeKeukeleire, D.; Hummer, K.; Heyerick, A.), s. 73-80. ISBN 978-90-6605-722-7. ISSN 0567-7572.
[ISHS International Humulus Symposium /2./. Ghent (BE), 01.09.2009-05.09.2009]
R&D Projects: GA ČR GA521/08/0740; GA MZe QH81052
Institutional research plan: CEZ:AV0Z50510513
Keywords : cluster analysis * expressed sequence tag microsatellite repeat (EST-SSR) * single nucleotide polymorphism (SNP)
Subject RIV: EB - Genetics ; Molecular Biology

New efficient and reliable expressed sequence tag microsatellite repeat (EST-SSR) molecular markers have been identified. Forty primer pairs for 29 gene-specific sequences were evaluated for identification and clustering in eight hops cultivars. Gene-specific markers based clustering was compared to clustering based on SSR and AFLP markers and on a combination of molecular markers. Gene-specific markers were showed to be ideal for DNA fingerprinting, for control of authenticity and purity of varieties, for evaluation of genetic diversity in genetic resources, etc. They are readily applicable in breeding, genetic linkage mapping and quantitative trait loci (QTL) analysis.
Permanent Link: http://hdl.handle.net/11104/0180410