Polyamine metabolism during the cell cycle of synchronized tobacco BY-2 cell line

0335900 - ÚEB 2010 RIV FR eng J - Journal Article
Gemperlová, Lenka - Cvikrová, Milena - Fischerová, Lucie - Binarová, Pavla - Fischer, L. - Eder, Josef
Polyamine metabolism during the cell cycle of synchronized tobacco BY-2 cell line.
[Metabolismus polyaminů v průběhu buněčného cyklu synchronní buněčné kultury tabáku BY-2.]
Plant Physiology and Biochemistry. Roč. 47, č. 7 (2009), s. 584-591. ISSN 0981-9428
R&D Projects: GA AV ČR IAA500200719
Institutional research plan: CEZ:AV0Z50380511; CEZ:AV0Z50200510
Keywords : ADC * Cell cycle * DAO
Subject RIV: EF - Botanics
Impact factor: 2.485, year: 2009

The time courses of the contents of free, soluble and insoluble polyamine (PA) conjugates, PA biosynthetic and catabolic enzyme activities and mRNA levels of PA biosynthetic genes were monitored during the cell cycle of synchronized tobacco BY-2 cell line (Nicotiana tabacum L. cv. Bright Yellow 2). Progression through the cell cycle was characterized by specific biphasic changes of PA levels. The first, moderate peak in the amount of free PAS coincided with the S-phase. After a transient decline in G2 phase the contents of free PAS increased rapidly and peaked again during G2/M interface. Then sharply decreased with the minimum at the end of mitosis and during M/G1 transition and started to rise again with the next replication phase. Levels of PA soluble conjugates paralleled those of the free forms. Biosynthetic enzyme activities followed the biphasic manner analogous to the levels of free PAS and seemed to be regulated on both transcriptional and (post)translational level.

Na regulaci vnitrobuněčné hladiny metabolicky aktivních volných polyaminů během buněčného cyklu tabákové kultury BY-2 se podílí: (i) aktivity biosyntetických enzymů polyaminů, které jsou regulovány na úrovni transkripce i na úrovni (post)translační, (ii) oxidativní dekarboxylace putrescinu během G2-fáze a v pozdní mitóze, (iii) konjugace polyaminů s hydroxyskořicovými kyselinami v oblasti G2/M a v průběhu mitózy.
Permanent Link: http://hdl.handle.net/11104/0180246