Focal Adhesion Kinase functions as an Akt downstream target in migration of colorectal cancer cells

0335669 - ÚMG 2010 RIV US eng J - Journal Article
Turečková, Jolana - Vojtěchová, Martina - Krausová, Michaela - Šloncová, Eva - Kořínek, Vladimír
Focal Adhesion Kinase functions as an Akt downstream target in migration of colorectal cancer cells.
Translational Oncology. Roč. 2, č. 4 (2009), s. 281-290. ISSN 1936-5233. E-ISSN 1936-5233
R&D Projects: GA ČR GA204/06/1658; GA MŠMT 2B06077
Institutional research plan: CEZ:AV0Z50520514
Keywords : FAK * migration * invasion
Subject RIV: EB - Genetics ; Molecular Biology

Migration plays a crucial role in cancer cell invasion and metastasis. The focus of this study is the collaboration of Akt, FAK and Src kinases in migration and invasion of colorectal cancer cells. We show that all three kinases can be found in one protein complex but the interaction between Akt and Src is indirect and mediated by FAK. Interestingly, induced Akt signaling causes an increased tyrosine phosphorylation of FAK but this increase is attenuated by Src inhibitor SU6656. We also show that active Akt stimulates cell migration, but this phenomenon is blocked by FAK knockdown or by inhibition of Src kinase. In addition, all three kinases appeared crucial for successful invasion of colorectal cancer cells. Alltogether, our data suggest the mechanism how the pathway can affect migration of colorectal adenocarcinoma cells. Since FAK acts downstream of Akt, our results imply FAK kinase as a potential key molecule during progression of tumors with active PI3-K/Akt signaling.
Permanent Link: http://hdl.handle.net/11104/0180069