Borrelia carolinensis sp. nov., a new (14th) member of the Borrelia burgdorferi Sensu Lato complex from the southeastern region of the United States

0335285 - BC 2010 RIV US eng J - Journal Article
Rudenko, Natalia - Golovchenko, Maryna - Grubhoffer, Libor - Oliver, J. H., Jr.
Borrelia carolinensis sp. nov., a new (14th) member of the Borrelia burgdorferi Sensu Lato complex from the southeastern region of the United States.
Journal of Clinical Microbiology. Roč. 47, č. 1 (2009), s. 134-141. ISSN 0095-1137. E-ISSN 1098-660X
R&D Projects: GA MŠMT(CZ) LC06009; GA ČR(CZ) GA524/06/1479
Institutional research plan: CEZ:AV0Z60220518
Keywords : multilocus sequence analysis * B. burgdorferi sl complex * new borrelia species * Borrelia carolinensis
Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine
Impact factor: 4.162, year: 2009

Approximately 118 Borrelia isolates were cultured from a variety of rodents, birds, and ticks collected in the southern United States. In addition to a highly diverse group of Borrelia bissettii strains and a homogenous group of Borrelia burgdorferi ss strains, a group of 16 isolates with unusual characteristics was found. The isolates were cultured from ear biopsy samples of the rodents trapped at five localities in South Carolina. Unique RFLP patterns of the rrf-rrl intergenic spacer region and fla gene were recognized. Unique signature nt were found in the 16S rRNA gene. A phylogenetic analysis shows that the 16 new isolates cluster together but separately from the other species in the B. burgdorferi sl complex. Our data strongly support the recognition of the 16 isolates as a new B. burgdorferi sl species. We proposed to name this genospecies Borrelia carolinensis.
Permanent Link: http://hdl.handle.net/11104/0179792