Molecular detection of Borrelia bissettii DNA in serum samples from patients in the Czech Republic with suspected borreliosis

0335284 - BC 2010 RIV NL eng J - Journal Article
Rudenko, Natalia - Golovchenko, Maryna - Růžek, Daniel - Piskunova, N. - Mallátová, N. - Grubhoffer, Libor
Molecular detection of Borrelia bissettii DNA in serum samples from patients in the Czech Republic with suspected borreliosis.
FEMS Microbiology Letters. Roč. 292, č. 2 (2009), s. 274-281. ISSN 0378-1097. E-ISSN 1574-6968
R&D Projects: GA MŠMT(CZ) LC06009; GA ČR GD524/03/H133; GA ČR(CZ) GA524/06/1479
Institutional research plan: CEZ:AV0Z60220518
Keywords : Borrelia bissettii in human serum * causative agent * co-infection * co-detection of different species * phylogenetic analysis
Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine
Impact factor: 2.199, year: 2009

Until recently, 3 spirochete genospecies were considered to be the causative agents of Lyme borreliosis (LB) in Europe: Borrelia burgdorferi sensu stricto, B. afzelii and B. garinii. However, the DNA of B. valaisiana, B. lusitaniae, B. spielmanii and B. bissettii has already been detected in samples of human origin, or the spirochetes were isolated from the patients with symptoms of LB. Molecular analysis of 12 selected serum samples collected in the regional hospital confirmed the presence of B. bissettii DNA in cases of single and multiple infection in patients with symptomatic borreliosis or chronic borrelial infection. The presence of B. bissettii as a single strain in patients provides strong support of the fact that B. bissettii might be a causative agent of the disease. Here we present additional molecular data supporting the involvement of B. bissettii in LB in Europe.
Permanent Link: http://hdl.handle.net/11104/0179791