Insects Pathogens: Molecular Approaches and Techniques

0335280 - BC 2010 RIV GB eng M - Monography Chapter
Oborník, Miroslav
Identification and Diagnostics of Entomopathogenic Protozoa.
Insects Pathogens: Molecular Approaches and Techniques. Wallingford: CAB International, 2009 - (Stock, S.; Vandenberg, J.; Glazer, I.; Boemare, N.), s. 101-128. ISBN 978-1-84593-478-1
Institutional research plan: CEZ:AV0Z60220518
Keywords : entomopathogenic protozoa * diagnostics * strains specific PCR
Subject RIV: EB - Genetics ; Molecular Biology

Molecular methods allow us to recognize and identify target organisms from a variety of environments including laboratory cultures, host tissues or environmental samples. Ascending progress on the field of molecular evolution during last 15 years greatly influenced high order taxonomy. It has been shown that the proposed kingdom Protozoa comprising unicellular heterotrophic eukaryotes is polyphyletic in its nature and its taxonomic relevance is no longer sustainable. Here I show the two up-to-date proposals dividing eukaryotic organisms into five and six major groups (kingdoms) respectively. These are currently the most accepted views on protist and eukaryotic high order taxonomy referred by Simpson and Roger (2004) and Keeling et al., (2005). This chapter follows methods for molecular identification of Protozoa colonizing insects and their molecular diagnostics.
Permanent Link: http://hdl.handle.net/11104/0179787