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Comparison of Metallothionein Detection by Using Brdicka Reaction and Enzyme-Linked Immunosorbent Assay Employing Chicken Yolk Antibodies

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    0335259 - ÚŽFG 2010 RIV DE eng J - Journal Article
    Křížková, S. - Bláhová, P. - Nakielna, J. - Fabrik, I. - Adam, V. - Eckschlager, T. - Beklová, M. - Svobodová, Z. - Horák, Vratislav - Krížek, R.
    Comparison of Metallothionein Detection by Using Brdicka Reaction and Enzyme-Linked Immunosorbent Assay Employing Chicken Yolk Antibodies.
    Electroanalysis. Roč. 21, č. 23 (2009), s. 2575-2583. ISSN 1040-0397. E-ISSN 1521-4109
    Institutional research plan: CEZ:AV0Z50450515
    Keywords : Metallothionein * Differential pulse voltammetry * ELISA
    Subject RIV: CG - Electrochemistry
    Impact factor: 2.630, year: 2009

    In this study, two methods for metallothioneins determination in a biological sample were compared. Human and pig blood serum samples and liver and kidney samples from carps (Cyprinus carpio) were analyzed by enzyme-linked immunosorbent assay and differential pulse voltammetry Brdicka´s reaction. The results obtained by these two methods were in good agreement. The correlation coefficients were 0.90 for fish liver and kidney samples, 0.91 for pig blood serum and 0.93 for human blood serum.
    Permanent Link: http://hdl.handle.net/11104/0179770

     
     
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