Photochemical and photoelectrochemical quenching of chlorophyll fluorescence in photosystem II

0335115 - MBÚ 2010 RIV NL eng J - Journal Article
Vredenberg, W. - Durchan, M. - Prášil, Ondřej
Photochemical and photoelectrochemical quenching of chlorophyll fluorescence in photosystem II.
[Fotochemické a fotoelektrochemické zhášení fluorescence chlorofylu ve Fotosystému II.]
Biochimica Et Biophysica Acta-Bioenergetics. Roč. 1787, č. 12 (2009), s. 1468-1478. ISSN 0005-2728. E-ISSN 1879-2650
R&D Projects: GA ČR GA206/08/1683
Institutional research plan: CEZ:AV0Z50200510
Keywords : Chlorophyll a fluorescence * Photosystem II * Heterogeneity
Subject RIV: EE - Microbiology, Virology
Impact factor: 3.688, year: 2009

This paper deals with kinetics and properties of variable fluorescence in leaves and thylakoids upon excitation with low intensity multi-turnover actinic light pulses corresponding with an excitation rate of about 10 Hz. These show a relatively small and amply documented rise in the sub-s time range towards the plateau level Fpl followed by a delayed and S-shaped rise towards a steady state level Fm which is between three and four fold the initial dark fluorescence level Fo. Properties of this retarded slow rise are i) rate of dark recovery is (1–6 s)−1, ii) suppression by low concentration of protonophores, iii) responsiveness to complementary single turnover flash excitation with transient amplitude towards a level Fm which is between five and six fold the initial dark fluorescence level Fo and iv) in harmony with and quantitatively interpretable in terms of a release of photoelectrochemical quenching controlled by the trans-thylakoid proton pump powered by the light-driven Q cycle

Práce se zabývá analýzou variabilní fluorescence chlorofylu. Ukazuje, že nárůst fluorescence má příšiny jak fotochemické, tak elektrofotochemické. Je představen model, který uspokojivě popisuje sledované jevy
Permanent Link: http://hdl.handle.net/11104/0179680