The effects of selected flavonoids on cytochromes P450 in rat liver and small intestine

0334401 - MBÚ 2010 RIV SK eng J - Journal Article
Křížková, J. - Burdová, K. - Stiborová, M. - Křen, Vladimír - Hodek, P.
The effects of selected flavonoids on cytochromes P450 in rat liver and small intestine.
Interdisciplinary Toxicology. Roč. 2, č. 3 (2009), s. 201-204. ISSN 1337-6853
R&D Projects: GA ČR GD305/09/H008
Institutional research plan: CEZ:AV0Z50200510
Keywords : flavonoids * cytochrome p450 * small intestine
Subject RIV: EE - Microbiology, Virology

Flavonoids, as foreign compounds (xenobiotics), have great potential to modulate the activity of cytochrome P450s (CYPs), xenobiotic-metabolizing enzymes involved in the activation and detoxification of food and environmental carcinogens. Thus, the aim of this study was to investigate the effects of model glycosylated and deglycosylated flavonoids on CYPs in rat liver and small intestine, as the two main organs responsible for xenobiotic metabolism, after p.o. administration by gastric gavages. The effects of two glycosylated flavonoids (isoquercitrin, rutin) and their aglycone (quercetin) on CYPs were determined using Western blotting technique and specific activity assays with alkyl-resorufin derivatives. In liver microsomes, a considerable increase of all the measured marker activities (EROD, MROD, PROD) was observed only after isoquercitrin treatment. Obtained data demonstrate the different effects of flavonoid glycosides and aglycone on CYP expression in rat liver and small intestine
Permanent Link: http://hdl.handle.net/11104/0179146