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Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases

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    0334373 - ÚOCHB 2010 RIV GB eng J - Journal Article
    Macíčková-Cahová, Hana - Hocek, Michal
    Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases.
    Nucleic Acids Research. Roč. 37, č. 22 (2009), s. 7612-7622. ISSN 0305-1048. E-ISSN 1362-4962
    R&D Projects: GA ČR GA203/09/0317; GA MŠMT LC512
    Institutional research plan: CEZ:AV0Z40550506
    Keywords : functionalized DNA * restriction endonucleases * DNA polymerase * modified adenosines
    Subject RIV: CC - Organic Chemistry
    Impact factor: 7.479, year: 2009

    A set of base-modified 2'-deoxyadenosine derivatives was incorporated to diverse DNA sequences by primer extension and the influence of the modification on cleavage by diverse restriction endonucleases was studied. While 8-substituted adenine derivatives were well tolerated by the enzymes, the presence of 7-substituted 7-deazaadenine in the recognition sequence resulted in blocking of cleavage by some enzymes depending on the nature and size of the 7-substituent. The results are useful both for protection of some sequences from cleavage and for manipulation of functionalized DNA by restriction cleavage.
    Permanent Link: http://hdl.handle.net/11104/0179124

     
     
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