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Identification of a single peridinin sensing Chl-a excitation in reconstituted PCP by crystallography and spectroscopy
- 1.0334361 - BC 2010 RIV US eng J - Journal Article
Schulte, T. - Niedzwiedzki, D. - Birge, R.R. - Hiller, R.G. - Polívka, Tomáš - Hofmann, E. - Frank, H.A.
Identification of a single peridinin sensing Chl-a excitation in reconstituted PCP by crystallography and spectroscopy.
Proceedings of the National Academy of Sciences of the United States of America. Roč. 106, č. 49 (2009), s. 20764-20769. ISSN 0027-8424. E-ISSN 1091-6490
Institutional research plan: CEZ:AV0Z50510513
Keywords : light harvesting * transient absorption spectroscopy * protein crystallography
Subject RIV: BO - Biophysics
Impact factor: 9.432, year: 2009
Combination of molecular biology, crystallography and spectroscopy allowed to identify structural and spectroscopic properties of a specific mutant of the PCP complex, which significantly changes absorption spectrum. Structure of this mutant was determined to 1.5 Å resolution and specific changes in absorption and transient absorption spectra were identified as due to electrochromic shift of perdinin Per-614 that senses the excited chlorophyll nearby.
Permanent Link: http://hdl.handle.net/11104/0179116
Number of the records: 1