Proving tumour cells by acute nutritional/energy deprivation as a survival threat: a task for microscopy

0334131 - ÚMG 2010 RIV GR eng J - Journal Article
Janečková, H. - Veselý, Pavel - Chmelík, R.
Proving tumour cells by acute nutritional/energy deprivation as a survival threat: a task for microscopy.
Anticancer Research. Roč. 29, č. 6 (2009), s. 2339-2345. ISSN 0250-7005. E-ISSN 1791-7530
Institutional research plan: CEZ:AV0Z50520514
Keywords : tumour cell * nutritional deprivation * energy deprivation * cell survival * cell death * digital holographic microscopy * dynamic phase difference
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.428, year: 2009

Malignant cells appear to possess a special aptitude for survival. We attempted to prove this in vitro by acute nutritional and energy deprivation as a survival threat. A PBS survival test in cell culture allowed static observations. These were supplemented by classic and quantitative phase-contrast time-lapse microscopy. From one normal and four neoplastic cell populations, no cells survived 77 hours of exposure to PBS. Only G3S2 derived from a human breast carcinoma survived 60 hrs. Cells in sparse culture were more vulnerable than those in dense. Epithelial cells were more vigorous than mesenchymal. Cells of greater malignancy resisted longer. Evaluation in culture as detailed by digital holographic microscopy (DHM) revealed increased compactness of the intracellular mass motility from normal to metastasizing mesenchymal cells, thus reaching the level of epithelial G3S2 cells. PBS survival test with DHM opens a new approach to studying the structural integrity of neoplastic cells.
Permanent Link: http://hdl.handle.net/11104/0178947