Specificity of anti-saliva immune response in mice repeatedly bitten by Phlebotomus sergenti

0334103 - ÚMG 2010 RIV GB eng J - Journal Article
Drahota, J. - Lipoldová, Marie - Volf, P. - Rohoušová, Iva
Specificity of anti-saliva immune response in mice repeatedly bitten by Phlebotomus sergenti.
Parasite immunology. Roč. 31, č. 12 (2009), s. 766-770. ISSN 0141-9838. E-ISSN 1365-3024
R&D Projects: GA MŠMT(CZ) LC06009
Grant - others:GA ČR(CZ) GP206/06/P015
Institutional research plan: CEZ:AV0Z50520514
Keywords : anti-saliva IgG * cellular response * Phlebotomus sergenti
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.014, year: 2009

Sand flies transmit parasites of genus Leishmania, the causative agents of leishmaniases. Experimental hosts repeatedly exposed to sand fly saliva can control Leishmania infection. Cell-mediated anti-saliva immune response is most likely responsible for this protective effect; however, there is no study concerning its antigenic specificity. In this study, splenocytes from mice exposed to Phlebotomus sergenti bites were challenged ex vivo with saliva from three different sand flies. Mice bitten by P. sergenti had higher proliferation to homologous antigen than splenocytes from naive mice. Splenocytes from P. sergenti bitten mice as well as anti-P. sergenti antibodies partially cross-reacted with P. papatasi saliva. In contrast, no crossreactivity was found with P. arabicus saliva. Our data indicate that both cellular and humoral immunity react in a species-specific manner and the unique transmission-blocking vaccine would be required for each vector – Leishmania combination.
Permanent Link: http://hdl.handle.net/11104/0178926