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Recovery of tubulin functions after freeze-dying in the presence of trehalose
- 1.0334042 - ÚMG 2010 RIV US eng J - Journal Article
Dráberová, Eduarda - Sulimenko, Vadym - Sulimenko, Tetyana - Böhm, K. J. - Dráber, Pavel
Recovery of tubulin functions after freeze-dying in the presence of trehalose.
Analytical Biochemistry. Roč. 397, č. 1 (2010), s. 67-72. ISSN 0003-2697. E-ISSN 1096-0309
R&D Projects: GA AV ČR KAN200520701; GA MŠMT LC545
Institutional research plan: CEZ:AV0Z50520514
Keywords : Tubulin * Freeze-drying * Stability
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.236, year: 2010
We describe a novel method for freeze-drying of assembly-competent tubulin in the presence of a nonreducing sugar trehalose. Even after prolonged storage at ambient temperature, rehydrated tubulin is capable of binding antimitotic drugs and assembling to microtubules that bind microtubule-associated proteins in the usual way.
Permanent Link: http://hdl.handle.net/11104/0178877
Number of the records: 1