Silencing CENPF in bovine preimplantation embryo induces arrest at 8-cell stage

0334003 - ÚŽFG 2010 RIV GB eng J - Journal Article
Toralová, Tereza - Šušor, Andrej - Němcová, Lucie - Kepková, Kateřina - Kaňka, Jiří
Silencing CENPF in bovine preimplantation embryo induces arrest at 8-cell stage.
Reproduction. Roč. 138, č. 5 (2009), s. 783-791. ISSN 1470-1626
R&D Projects: GA ČR GA523/06/1226; GA ČR(CZ) GD204/05/H023
Institutional research plan: CEZ:AV0Z50450515
Keywords : CENPF * bovine embryo * preimplantation development
Subject RIV: GI - Animal Husbandry ; Breeding
Impact factor: 2.579, year: 2009

The paper deals with the role of CENPF/mitosin during preimplantation development of cattle. We focused on monitoring of developmental competence of bovine embryos after injection of CENPF dsRNA into the zygotes. An average decrease of CENPF mRNA abundance by 94.9% or more and an extensive decline in immunofluorescence staining intensity was detected relative to controls. There was no disparity between the groups in the developmental competence before the 8-cell stage, where embryonic genome activation (EGA) in bovines takes place. However, the developmental competence rapidly decreased then and only 28.1% of CENPF dsRNA injected 8-cell embryos were able to develop further (controls 72.0%). We have further shown that in bovine post-EGA embryos CENPF protein is expressed in the same manner as in somatic cells. However, the protein in pre-EGA embryos is likely not degraded at the end of cell cycle, which enables the development of embryos till 8-cell stage.
Permanent Link: http://hdl.handle.net/11104/0178848