Controlled dehydration of a biological sample using an alternative form of environmental SEM

0333986 - ÚPT 2010 RIV GB eng J - Journal Article
Neděla, Vilém
Controlled dehydration of a biological sample using an alternative form of environmental SEM.
Journal of Microscopy. Roč. 237, č. 1 (2010), s. 7-11. ISSN 0022-2720. E-ISSN 1365-2818
Institutional research plan: CEZ:AV0Z20650511
Keywords : biological sample * dehydration * environmental SEM * AQUASEM II * hydration system
Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering
Impact factor: 1.872, year: 2010

In this study a non-conductive biological sample is observed free of charging artefacts when placed on a cooled Peltier stage in the specimen chamber of an alternative form of the environmental scanning electron microscope, equipped with a specially designed hydration system. This system was used to create dynamically changing surrounding conditions leading to controlled dehydration of the sample enabling us to visualize the topographical structure of a rat tongue in the transition region between the liquid and the gas state of water in the microscope specimen chamber.
Permanent Link: http://hdl.handle.net/11104/0178835