Head and neck squamous cancer stromal fibroblasts produce growth factors influencing phenotype of normal human keratinocytes

0333778 - ÚMG 2010 RIV DE eng J - Journal Article
Strnad, Hynek - Lacina, L. - Kolář, Michal - Čada, Z. - Vlček, Čestmír - Dvořánková, B. - Betka, J. - Plzák, J. - Chovanec, M. - Šáchová, Jana - Valach, J. - Urbanová, Markéta - Smetana, K. Jr.
Head and neck squamous cancer stromal fibroblasts produce growth factors influencing phenotype of normal human keratinocytes.
Histochemistry and Cell Biology. Roč. 133, č. 2 (2010), s. 201-211. ISSN 0948-6143. E-ISSN 1432-119X
R&D Projects: GA MŠMT 2B06106
Grant - others:GA ČR(CZ) GP304/08/P175
Institutional research plan: CEZ:AV0Z50520514
Keywords : Cancer microenvironment * Epithelial–mesenchymal interaction * Cancer-associated fibroblasts
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 4.727, year: 2010

Epithelial–mesenchymal interaction between stromal fibroblasts and cancer cells influences the functional properties of tumor epithelium, including the tumor progression and spread. We compared fibroblasts prepared from stroma of squamous cell carcinoma and normal dermal fibroblasts concerning their biological activity toward normal keratinocytes assessed by immunocytochemistry and profiling of gene activation for growth factors/cytokines by microarray chip technology. IGF-2 and BMP-4 were determined as candidate factors responsible for tumor-associated fibroblast activity that influences normal epithelia. This effect was confirmed by addition of recombinant IGF-2 and BMP4, respectively, to the culture medium. This hypothesis was also verified by inhibition experiments where blocking antibodies were employed in the medium conditioned by cancer-associated fibroblast. Presence of these growth factors was also detected in tumor samples.
Permanent Link: http://hdl.handle.net/11104/0178688