GAR22: A novel target gene of thyroid hormone receptor causes growth inhibition in human erythroid cells

0333660 - ÚMG 2010 RIV US eng J - Journal Article
Gamper, I. - Koh, K.-R. - Ruau, D. - Ullrich, K. - Bartůňková, Jana - Piroth, D. - Hacker, C. - Bartůněk, Petr - Zenke, M.
GAR22: A novel target gene of thyroid hormone receptor causes growth inhibition in human erythroid cells.
Experimental Hematology. Roč. 37, č. 5 (2009), s. 539-548. ISSN 0301-472X. E-ISSN 1873-2399
R&D Projects: GA MŠMT(CZ) LC06077
Institutional research plan: CEZ:AV0Z50520514
Keywords : Thyroid hormone receptor * GAR22 * erythropoiesis
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.106, year: 2009

Thyroid hormone receptors (TRs) are ligand-dependent transcription factors with a major impact on erythroid cell development. Here we investigated TR activity on red cell gene expression using a genome-wide approach with DNA microarrays and identified TR target genes. Ligand-activated TR effectively accelerated red cell progenitor differentiation in vitro concomitantly with inducing growth arrest. We demonstrate that activated TR induced specific gene expression patterns of up- or down-regulated genes, including distinct clusters associated with accelerated differentiation in response to treatment. Mining for T3 induced genes identified BTEB1 and GAR22 as TR target genes. BTEB1 is a known TR target gene while GAR22, initially identified as a putative tumor suppressor, represents a novel TR target gene. We demonstrate that ectopic GAR22 expression in red cell progenitors lengthens the cell cycle and causes growth inhibition, but leaves red cell gene expression unaffected.
Permanent Link: http://hdl.handle.net/11104/0178599