Number of the records: 1
Insect cold tolerance and repair of chill-injury at fluctuating thermal regimes: Role of 70kDa heat shock protein expression
- 1.0333184 - BC 2010 RIV GB eng J - Journal Article
Tollarová-Borovanská, Michaela - Lalouette, L. - Košťál, Vladimír
Insect cold tolerance and repair of chill-injury at fluctuating thermal regimes: Role of 70kDa heat shock protein expression.
[Chladová odolnost hmyzu a oprava poškození chladem: Úloha šokových proteinů z rodiny HSP 70kDa.]
Cryo-letters. Roč. 30, č. 5 (2009), s. 312-319. ISSN 0143-2044. E-ISSN 1742-0644
R&D Projects: GA ČR GA206/07/0269
Institutional research plan: CEZ:AV0Z50070508
Keywords : insect * cold tolerance * heat shock proteins
Subject RIV: ED - Physiology
Impact factor: 1.074, year: 2009
Expression of heat shock proteins has been proposed as an underlying mechanism of increased cold tolerance in insects exposed to fluctuating thermal regimes (FTRs) in comparison to constant low temperatures (CLTs). We found that the levels of /Pahsp70/ mRNA increase by up to 3 orders in the linden bugs, /Pyrrhocoris apterus/ exposed to FTR -5^(o)C (22h)/ 25^(o)C (2h). The 2h-long warm pulses, however, were not sufficient for accumulation of PaHSP70 protein and thus no significant difference in expression of PaHSP70 protein was detected between FTR and CLT regimes. Hence, we conclude that the accumulation of PaHSP70 protein is not the mechanism underlying the increased cold tolerance in /P. apterus/ at the particular FTR used in this study. The relevance of some other possible mechanisms is discussed.
Četnost mRNA transkriptů genu /Pahsp70/ se zvýšila o tři řády během vystavení ploštice /Pyrrhocoris apterus/ chladu [FTR - fluktuační termální režim: -5^(o)C (22 hod)/ 25^(o)C (2 hod)]. Relativně krátká perioda vystavení vyšši teplotě během FTR (2 hod) byla však nedostatečná pro zajištění exprese a akumulace kódovaného proteinu. Přes výrazný rozdíl v expresi genu proto nebyl pozorován žádný rozdíl v expresi proteinu mezi FTR a vystavením v konstatní nizké teplotě.
Permanent Link: http://hdl.handle.net/11104/0178236
Number of the records: 1