Nerve Agents Assay Using Cholinesterase Based Biosensor

0332920 - ÚOCHB 2010 RIV DE eng J - Journal Article
Pohanka, M. - Dobeš, Petr - Drtinová, L. - Kuča, K.
Nerve Agents Assay Using Cholinesterase Based Biosensor.
Electroanalysis. Roč. 21, č. 10 (2009), s. 1177-1182. ISSN 1040-0397. E-ISSN 1521-4109
Grant - others:GA MO(CZ) OVUOFVZ200807
Institutional research plan: CEZ:AV0Z40550506
Keywords : organophosphate * biosensor * acetylcholinesterase
Subject RIV: CF - Physical ; Theoretical Chemistry
Impact factor: 2.630, year: 2009

Electrochemical biosensor based on electric eel acetylcholinesterase (AChE) (EC 3.1.1.7) was performed for assay of nerve agents – tabun, sarin, soman, cyclosarin, and VX. The biosensor used AChE as biorecognition element. The presence of nerve agents was accompanied by a strong inhibition of AChE activity. Enzyme activity is easily measurable by electrochemical oxidation of thiocholine created from acetylthiocholine (ATChCl) by AChE-catalyzed hydrolysis. The tested nerve agents were successfully assayed. The best limits of detection were achieved for sarin (5.88×10-10 M) and VX (8.51×10-10 M) after one-minute assay. The biosensor was found long term stable at low as well as laboratory temperature.
Permanent Link: http://hdl.handle.net/11104/0178034