Comparison of HPLC/MS and MALDI-MS for characterizing triacylglycerols in insects: Specie-specific composition of lipids in fat bodies of bumblebee males

0332852 - ÚOCHB 2010 RIV NL eng J - Journal Article
Kofroňová, Edita - Cvačka, Josef - Vrkoslav, Vladimír - Hanus, Robert - Jiroš, Pavel - Kindl, Jiří - Hovorka, Oldřich - Valterová, Irena
Comparison of HPLC/MS and MALDI-MS for characterizing triacylglycerols in insects: Specie-specific composition of lipids in fat bodies of bumblebee males.
Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. Roč. 877, č. 30 (2009), s. 3878-3884. ISSN 1570-0232. E-ISSN 1873-376X
R&D Projects: GA ČR GA203/09/0139; GA ČR GA203/09/1446; GA MŠMT 2B06007
Institutional research plan: CEZ:AV0Z40550506
Keywords : Bumblebee * fat body * triacylglycerols * HPLC/APCI-MS * MALDI-MS
Subject RIV: CC - Organic Chemistry
Impact factor: 2.777, year: 2009

Two mass spectrometric methods for analysing triacylglycerols (HPLC/APCI-MS and MALDI-MS) were used and compared in terms of the relevance of the data for further biostatistical evaluation. While MALDI-MS is simpler and significantly faster, the time-consuming and labour-intensive HPLC/APCI-MS provides more complete information about the lipid components. The compositions of triacylglycerols in the fat bodies of males in 11 bumblebee species (Bombus terrestris, B. lucorum, B. lapidarius, B. pratorum, B. sylvarum, B. ruderatus, B. pomorum, B. subterraneus, B. campestris, B. bohemicus, and B. rupestris) were found to be species-specific.
Permanent Link: http://hdl.handle.net/11104/0177980