Expression of Tumor Necrosis Factor Alpha-Included Protein 6 Messenger RNA in Porcine Preovulatory Ovarian Follicles

0332768 - ÚŽFG 2010 RIV JP eng J - Journal Article
Nagyová, Eva - Němcová, Lucie - Procházka, Radek
Expression of Tumor Necrosis Factor Alpha-Included Protein 6 Messenger RNA in Porcine Preovulatory Ovarian Follicles.
Journal of Reproduction and Development. Roč. 55, č. 3 (2009), s. 231-235. ISSN 0916-8818
R&D Projects: GA ČR GA523/08/0111
Institutional research plan: CEZ:AV0Z50450515
Keywords : Preovulatory Ovarian Follicles * TNFAIP6 * female fertility
Subject RIV: ED - Physiology
Impact factor: 1.697, year: 2009

Tumor necrosis factor alpha -induced protein 6 (TNFAIP6) is essential for formation of the cumulus extracellular matrix and female fertility. We studied its expression in porcine preovulatory follicles. The levels of TNFAIP6 mRNA were low in follicles on day 12 and day 15 of the estrous cycle and at 66 h after eCG stimulation but were significantly increased at 4 h after hCG. The high level of TNFAIP6 expression was maintained until 16 h after hCG stimulation and gradually decreased at 24 and 32 h after hCG. During in vitro culture, FSH/LH-induced TNFAIP6 mRNA was expressed in both OCCs and MGCs in a similar temporal pattern as seen in vivo. We conclude that TNFAIP6 expression in the pig, like other species, increases in preovulatory follicles following the LH(hCG) surge. The OCC and MGC display similar patterns of TNFAIP6 expression under both in vivo and in vitro conditions.
Permanent Link: http://hdl.handle.net/11104/0177913