Association of ‘Candidatus Phytoplasma asteris’ with yellowing and phyllody of Plantago lanceolata

0331800 - BC 2010 RIV CZ eng J - Journal Article
Fránová, Jana - Šimková, Marie
Association of ‘Candidatus Phytoplasma asteris’ with yellowing and phyllody of Plantago lanceolata.
Folia Microbiologica. Roč. 54, č. 5 (2009), s. 469-472. ISSN 0015-5632. E-ISSN 1874-9356
R&D Projects: GA AV ČR(CZ) 1QS500510558
Institutional research plan: CEZ:AV0Z50510513
Keywords : symptoms * electron microscopy * molecular identification
Subject RIV: EE - Microbiology, Virology
Impact factor: 0.978, year: 2009

Long plantains with symptoms resembling those associated with phytoplasma infection were observed in southern Bohemia. TEM showed phytoplasmas in the sieve cells of affected plants. Association of phytoplasmas with the disease was confirmed by PCR using phytoplasma-specific universal ribosomal primers R16F2n/R16R2. The restriction profiles obtained from digestion of the PCR products with endonucleases AluI, HhaI, and MseI showed that the phytoplasmas infecting long plantains in the Czech Republic were indistinguishable from those belonging to the aster yellows group (subgroup 16SrI-B). Sequence analysis of 1748 bp of the ribosomal operon indicated that the closest related phytoplasma was that associated with ‘Rehmannia glutinosa var. purpurea’, originating also in Bohemia. This is the first report of the natural occurrence of ‘Candidatus Phytoplasma asteris’ in plants of Plantago lanceolata.
Permanent Link: http://hdl.handle.net/11104/0177222