Distribution of benthic phototrophs, sulfate reducers, and methanogens in two adjacent saltern evaporation ponds in Eilat, Israel

0331178 - BC 2010 RIV DE eng J - Journal Article
Sørensen, K. - Řeháková, Klára - Zapomělová, Eliška - Oren, A.
Distribution of benthic phototrophs, sulfate reducers, and methanogens in two adjacent saltern evaporation ponds in Eilat, Israel.
[Vertikální zonace bentických fototrofů, sulfátových reducentů a metanogenních bakterií ve dvou vybraných odsolovacích nádržích v Eilatu, Izrael.]
Aquatic Microbial Ecology. Roč. 56, 2-3 (2009), s. 275-284. ISSN 0948-3055. E-ISSN 1616-1564.
[GAP workshop /8./. Eilat, 30.03.2008-08.04.2008]
R&D Projects: GA ČR(CZ) GA206/06/0462; GA AV ČR(CZ) KJB600960703; GA AV ČR(CZ) 1QS600170504
Institutional research plan: CEZ:AV0Z60170517; CEZ:AV0Z60050516
Keywords : salterns * microbial community * molecular ecology * phototrohs * sulfate reducers * methanogens
Subject RIV: EH - Ecology, Behaviour
Impact factor: 1.743, year: 2009

The distribution of microorganisms was examined in evaporation ponds. The biogeochemistry of the ponds was examined using microsensors. These measurements showed that net rates of oxygen production/consumption were higher at the lower salinity. The distribution of phototrophs was studied by microscopy, which revealed differences between the 2 crusts. The relative amounts of Bacteria, Archaea, sulfate reducers and methanogens were studied by real-time quantitative PCR. Sulfate reducers and methanogens were detected only in the deepest part of the phototrophic zone. Sulfate reducers were most abundant in the zone just below the phototrophic layer. Methanogens were less abundant in higher salinity, and than sulfate reducers. Inter-pond and vertical variations in the composition of communities were characterized by DGGE analysis. The detected phylotypes were distributed in a distinct pattern in the crust according to both biogeochemical regimes and salinity.

Bylo studováno vertikální rozložení mikroorganismů v sedimentu odsolovacích nádrží v Eilatu pomocí molekulárních metod (real-PCR, DGGE) a klasické mikroskopie. Zároveň byly měřeny biochemické charakteristiky sedimentu pomocí mikrosensoru. Získané výsledky ukázaly rozdíly v druhovém složení fototrofů , sulfátových reducentů a metanogenních bakterií v jednotliových vrstvách sedimentu, které byly způsobeny biochemickými rozdíly v jednotlivých vrstvách sedimentu a rozdílnou salinitou prostředí. Sulfátoví reducenti a metanogenní bakterie byly přítomni hlavně v hlubších vrstvách a zároveň lépe rostly v nižšších koncetracích soli.
Permanent Link: http://hdl.handle.net/11104/0176773