Complementation analysis of triple gene block of Potato virus S (PVS) revealed its capability to support systemic infection and aphid transmissibility of recombinant Potato virus X

0330919 - BC 2010 RIV NL eng J - Journal Article
Matoušek, Jaroslav - Schubert, J. - Dědič, P.
Complementation analysis of triple gene block of Potato virus S (PVS) revealed its capability to support systemic infection and aphid transmissibility of recombinant Potato virus X.
Virus Research. Roč. 146, č. 1 (2009), s. 81-88. ISSN 0168-1702. E-ISSN 1872-7492
R&D Projects: GA MZe QF3109; GA AV ČR(CZ) 1QS500510558
Grant - others:EU Projekt FP7-REGPOT-2008-1 MOBITAG(XE) 229518
Institutional research plan: CEZ:AV0Z50510513
Keywords : Recombinant virus * Virus host range * Potato virus X * Potato virus S
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.563, year: 2009

Specific differences were found in triple gene block (TGB) sequences derived from isolates of ordinary Potato virus S (PVS-O) and Chenopodium-systemic (PVS-CS). In order to investigate functional divergence of PVS TGB variants, they were propagated in chimeric Potato virus X (PVX). Both PVS TGB variants partly complemented PVX TGB, but multiplied to lower titer than the wild-type PVX in N. benthamiana showing attenuated symptoms. From the propagation of recombinant PVX variants in different host and non-host plant species we assume there are additional determinants outside the TGB. Transmission by the aphid Myzus persicae in the recombinant virus was obtained by replacing PVX TGB by TGB from the PVS-CS isolate. These results show the potential function of Carlavirus TGB in aphid transmissibility and underlines the possible biological risks from certain recombinant virus variants.
Permanent Link: http://hdl.handle.net/11104/0176587