Estrogenic and androgenic activity of PCBs, their chlorinated metabolites and other endocrine disruptors estimated with two in vitro yeast assays

0330344 - MBÚ 2010 RIV NL eng J - Journal Article
Svobodová, Kateřina - Plačková, Martina - Novotná, V. - Cajthaml, Tomáš
Estrogenic and androgenic activity of PCBs, their chlorinated metabolites and other endocrine disruptors estimated with two in vitro yeast assays.
Science of the Total Environment. Roč. 407, č. 22 (2009), s. 5921-5925. ISSN 0048-9697. E-ISSN 1879-1026
R&D Projects: GA MŠMT LC06066; GA MŠMT 2B06156; GA ČR GA525/09/1058
Institutional research plan: CEZ:AV0Z50200510
Keywords : endocrine distruptors * yeast bioassays * triclosan
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.905, year: 2009

Investigations of environmental pollution by endocrine disrupting chemicals are now in progress. Up to now, several in vitro bioassays have been developed for evaluation of the endocrine disruptive activity; however, there is still a lack of comparative studies of their sensitivity. In this work comparison of the estrogen screening assay based on b-galactosidase expression and a bioluminiscent estrogen screen revealed differences in the sensitivity and specificity of the two tests. With the b-galactosidase screen a slight estrogen-like activity of Delor 103, a commercial mixture of PCB congeners, and a fungicide triclosan was measured whereas no activity was detected using the bioluminescent assay. A bioluminescent androgen test negated previously suggested androgenic potential of triclosan
Permanent Link: http://hdl.handle.net/11104/0176156