Early cytokinin response proteins and phosphoproteins of Arabidopsis thaliana identified by proteome and phosphoproteome profiling

0330091 - ÚIACH 2010 CZ eng A - Abstract
Černý, M. - Dyčka, Filip - Bobálová, Janette - Divíšková, E. - Koukalová, Š. - Brzobohatý, B.
Early cytokinin response proteins and phosphoproteins of Arabidopsis thaliana identified by proteome and phosphoproteome profiling.
[Změny v proteomu a fosfoproteomu v brzké odpovědi na cytokininy v Arabidopsis thaliana.]
ACPD 2009. Prague: Czech University of Life Sciences Prague, 2009. s. 34.
[ACPD 2009. Auxins and Cytokinins in Plant Development International Symposium. 10.07.2009-14.07.2009, Prague]
R&D Projects: GA AV ČR IAA600040701; GA MŠMT 1M06030
Institutional research plan: CEZ:AV0Z40310501
Keywords : phosphoproteins * Arabidopsis thaliana * cytokinin
Subject RIV: CB - Analytical Chemistry, Separation

We employed 2-DE followed by image analysis and MALDI-TOF-TOF MS to analyze early changes in steady-state protein levels and phosphorylation status of the proteome in cytokinin treated Arabidopsis seedlings. Effects of four principal cytokinins (trans-zeatin, benzylaminopurine, thidiazuron and 6-(γ,γ-Dimethylallylamino)purine) were compared.

V této práci byly sledovány změny proteinového a fosfoproteinového profilu rostliny Arabidopsis v odpovědi na cytokininy pomocí metod 2-DE, analýzou obrazu a MALDI-TOF-TOF MS
Permanent Link: http://hdl.handle.net/11104/0175957