G-protein activity in Percoll-purified plasma membranes, bulk plasma membranes, and low-density plasma membranes isolated from rat cerebral cortex

0329471 - FGÚ 2010 RIV US eng J - Journal Article
Bouřová, Lenka - Stöhr, Jiří - Lisý, Václav - Rudajev, Vladimír - Novotný, Jiří - Svoboda, Petr
G-protein activity in Percoll-purified plasma membranes, bulk plasma membranes, and low-density plasma membranes isolated from rat cerebral cortex.
[Aktivita G-proteinů v purifikovaných plazmatických membránách, většinové membránové části a nízkodezitních membránách izolovaných z kortexu potkana.]
Medical Science Monitor. Roč. 15, č. 4 (2009), BR111-BR122. ISSN 1234-1010
R&D Projects: GA MŠMT(CZ) LC554; GA MŠMT(CZ) LC06063; GA ČR(CZ) GA309/06/0121; GA AV ČR(CZ) IAA500110606
Institutional research plan: CEZ:AV0Z50110509
Keywords : rat cerebral cortex * plasma membrane * G-protein activity
Subject RIV: CE - Biochemistry
Impact factor: 1.543, year: 2009

This work describe a straightforward method for the isolation of clearly defined plasma membrane preparations from rat brain cortex - Percoll-purified plasma membranes, bulk of plasma membranes and low-density plasma membrane fragments. [35S]GTPγS binding experiments measured in the presence of high concentration of GDP succeeded in the resolution of the agonist-responsive component of total binding. The highest efficacy of baclofen agrees well with the role of GABA and GABA-B receptors in inhibitory signalling of the brain, the order of efficacy of different agonists of opioid receptors was DADLE DAMGO U-69593

Aktivita G-proteinů v purifikovaných plazmatických membránách, většinové membránové části a nízkodezitních membránách izolovaných z kortexu potkana
Permanent Link: http://hdl.handle.net/11104/0175497