Chiral analysis of anti-acquired immunodeficiency syndrome drug, 9-(R)-[2-(phosphonomethoxy)propyl]adenine (tenofovir), and related antiviral acyclic nucleoside phosphonates by CE using beta-CD as chiral selector

0329291 - ÚOCHB 2010 RIV DE eng J - Journal Article
Šolínová, Veronika - Kašička, Václav - Sázelová, Petra - Holý, Antonín
Chiral analysis of anti-acquired immunodeficiency syndrome drug, 9-(R)-[2-(phosphonomethoxy)propyl]adenine (tenofovir), and related antiviral acyclic nucleoside phosphonates by CE using beta-CD as chiral selector.
Electrophoresis. Roč. 30, č. 12 (2009), s. 2245-2254. ISSN 0173-0835. E-ISSN 1522-2683
R&D Projects: GA ČR(CZ) GA203/06/1044; GA ČR(CZ) GA203/08/1428; GA AV ČR 1QS400550501; GA MŠMT 1M0508
Institutional research plan: CEZ:AV0Z40550506
Keywords : antiviral drugs * capillary electrophoresis * enantioseparation
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 3.077, year: 2009

A new capillary electrophoretic method has been developed for chiral analysis of an important anti-AIDS drug, 9-(R)-[2-(phosphonomethoxy)propyl]adenine ((R)-PMPA, tenofovir), and six related antiviral acyclic nucleoside phosphonates using beta cyclodextrin (beta CD) as a chiral selector. Complete separations of (R,S)-enantiomers of PMPA with very good resolution (Rs = 1.50 – 3.64) were achieved within a short time (4 – 15 min) in 20 – 50 mM sodium borate or sodium tetraborate BGEs, pH 10.0, at 20 mg/mL concentration of beta CD.
Permanent Link: http://hdl.handle.net/11104/0175366