Lasioglossins: Three novel antimicrobial peptides from the venom of the eusocial bee Lasioglossum laticeps (Hymenoptera: Halictidae)

0329288 - ÚOCHB 2010 RIV DE eng J - Journal Article
Čeřovský, Václav - Buděšínský, Miloš - Hovorka, Oldřich - Cvačka, Josef - Voburka, Zdeněk - Slaninová, Jiřina - Borovičková, Lenka - Fučík, Vladimír - Bednárová, Lucie - Votruba, Ivan - Straka, J.
Lasioglossins: Three novel antimicrobial peptides from the venom of the eusocial bee Lasioglossum laticeps (Hymenoptera: Halictidae).
Chembiochem. Roč. 10, č. 12 (2009), s. 2089-2099. ISSN 1439-4227. E-ISSN 1439-7633
R&D Projects: GA ČR(CZ) GA203/08/0536
Institutional research plan: CEZ:AV0Z40550506
Keywords : lasioglossins * peptides * Lasioglossum laticeps
Subject RIV: CC - Organic Chemistry
Impact factor: 3.824, year: 2009

Three novel antimicrobial peptides H-Val-Asn-Trp-Lys-Lys-Val-Leu-Gly-Lys-Ile-Ile-Lys-Val-Ala-Lys-NH2 (LL-I), H-Val-Asn-Trp-Lys-Lys-Ile-Leu-Gly-Lys-Ile-Ile-Lys-Val-Ala-Lys-NH2 (LL-II) and H-Val-Asn-Trp-Lys-Lys-Ile-Leu-Gly-Lys-Ile-Ile-Lys-Val-Val-Lys-NH2 (LL-III) designated lasioglossins were identified in the venom of the wild be Lasioglossum laticeps. They possess high antimicrobial activity against both Gram-positive and -negative bacteria and low hemolytic activity. As indicated by CD and NMR spectroscopy, lasioglossins can adopt an amphipathic alpha-helical secondary structure in an anisotropic environment such as the bacterial cell membrane. NMR study confirmed that this alpha-helical structure is curved with a concave hydrophobic and convex hydrophilic side.
Permanent Link: http://hdl.handle.net/11104/0175364