Hormonal and cell division analyses in Watsonia lepida seedlings

0328779 - ÚEB 2010 RIV PT eng J - Journal Article
Ascough, G. D. - Novák, Ondřej - Pěnčík, Aleš - Rolčík, Jakub - Strnad, Miroslav - Erwin, J. E. - van Staden, J.
Hormonal and cell division analyses in Watsonia lepida seedlings.
Journal of Plant Physiology. Roč. 166, č. 14 (2009), s. 1497-1507. ISSN 0176-1617. E-ISSN 1618-1328
Institutional research plan: CEZ:AV0Z50380511
Keywords : Auxin * Cytokinin * Differentiation
Subject RIV: CE - Biochemistry
Impact factor: 2.500, year: 2009

The regeneration ability, cell division activity, auxin and cytokinin content of seedling regions and hypocotyl subsections of Watsonia lepida were studied. A total of 21 different cytokinins or conjugates were found. The greatest contribution to the cytokinin pool came from the biologically inactive cZRMP. Five different auxins were detected. Analysis of hypocotyl subsections (C1–C4) revealed that cell division was highest in subsection C2, although regeneration in vitro was significantly lower than in subsection C1. Anatomically, subsection C1 contains the apical meristem, and hence has meristematic cells that are developmentally plastic. In contrast, subsection C2 has cells that have recently exited the meristem and are differentiating. Despite high rates of cell division, cells in subsection C2 appear no longer able to respond to cues that promote proliferation in vitro. Auxin and cytokinin analyses of these subsections were conducted.
Permanent Link: http://hdl.handle.net/11104/0174999