Supercritical Fluid Fractionation for Thymoquinone Enrichment in Black Cumin and Savory Isolates

0328751 - ÚCHP 2010 RIV DE eng C - Conference Paper (international conference)
Sajfrtová, Marie - Karban, Jindřich - Klouček, P. - Malík, J. - Kokoška, L.
Supercritical Fluid Fractionation for Thymoquinone Enrichment in Black Cumin and Savory Isolates.
Abstracts. Stuttgard: Thieme Verlag, 2009 - (Hostettmann,, K.; Wolfender, J.), s. 1041-s.1041. ISSN 0032-0943. E-ISSN 1439-0221.
[International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research /57./. Geneva (CH), 16.08.2009-20.08.2009]
R&D Projects: GA MŠMT 2B06049; GA ČR GA525/08/1179
Institutional research plan: CEZ:AV0Z40720504
Keywords : supercritical * fractionation * thymoquinone
Subject RIV: CI - Industrial Chemistry, Chemical Engineering

This work is focused on the enhancement of TQ concentration in volatile oil using SFE of black cumin (Nigella sativa L.) seeds and savory (Satureja hortensis L.) aerial parts. Black cumin volatile oil is one of the richest sources of TQ, in savory, TQ is a minor component. Volatile compounds in extracts were determined by GC-MS and GC-FID. The composition is strongly influenced by extraction pressure and modifier content in CO2. The highest concentration of TQ in savory extracts (1.5 wt.%) was obtained at 28 MPa, 50°C, 4.7 wt.% of acetone. In case of black cumin, the use of mild conditions (12 MPa, 50°C, 0 wt. % acetone) allowed separation of the volatile oil from fatty oil. Concentration of 20 wt.% of TQ was reached using 3.7g CO2/g seeds.
Permanent Link: http://hdl.handle.net/11104/0174984